pTacNeo1.3 [CMCC 2297] (ATCC® 37688)

Applications: expression vectorshuttle vectorvector permitting construction of fusion proteins  /  Depositors: JC Hunter-Cevera

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Designations pTacNeo1.3 [CMCC 2297]
Depositors JC Hunter-Cevera
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.4499998092651370
Vector: pTacNeo1.3 (plasmid)
Promoters: Promoter tac
Construction: pBR322, TAC promoter, lacZ, modified APH(3')-I
Marker(s):ampR
Construct size (kb): 5.449999809265137
Features: marker(s): ampR
promoter: tac
replicon: pMB1
Applications
expression vector
shuttle vector
vector permitting construction of fusion proteins
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.45; HindIII--5.45; EcoRI--5.45; PvuII--4.0, 1.5; PstI--3.1, 2.35.
This is an expression vector capable of producing a beta-galactosidase: insert: APH(3')-I fusion protein.
The start codon of the modified APH(3')-I is followed by the eleventh codon (serine) of APH(3')-I. Codons 2-10 have been deleted.
The upstream translation of LacZ is out of frame with APH(3')-I.
Sequences from the TAA stop codon of APH(3')-I to the immediate downstream PvuII site encode a portion of Tn601. Sequences from the PvuII site to the PstI site are derived from Tn5.
The vector contains pBR322 (EcoRI-SphI), a TAC promoter, the first 8 amino-terminal codons of beta-galactosidase, and a gene encoding a modified aminoglycoside phosphotransferase (3')-I (APH(3')-I).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Jennie C Hunter-Cevera, personal communication

Shipped freeze-dried
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