Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 8.6999998092651370
Vector: YCp8cat (plasmid)
Promoters: Promoter none
Construction: YRp7-32cat (ATCC 37624)
, CEN6, URA3
Construct size (kb): 8.699999809265137
Features: insert detection: CAT
marker(s): ampR, TRP1, URA3
replicon: pMB1, ARS1
YC-type (centromeric) shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--8.8; BglII--6.7, 2.8; EcoRI--7.2, 1.45, 0.57.
The depositor recommends using YRp7-17camp (ATCC 37630)
as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624
through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 7285 bp BamHI/HindIII fragment of YCp8 containing URA3 and CEN6 was ligated to a 1415 bp BamHI/HindIII fragment of YRp7-32cat (ATCC 37624)
Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257