YCp8cat (ATCC® 37633)

Applications: YC-type (centromeric) shuttle vectorpromoter-cloning vectorshuttle vector  /  Depositors: H Feldmann

Permits and Restrictions

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Designations YCp8cat
Depositors H Feldmann
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 8.6999998092651370
Vector: YCp8cat (plasmid)
Promoters: Promoter none
Construction: YRp7-32cat (ATCC 37624), CEN6, URA3
Construct size (kb): 8.699999809265137
Features: insert detection: CAT
marker(s): ampR, TRP1, URA3
promoter: none
replicon: pMB1, ARS1
centromere: CEN6
YC-type (centromeric) shuttle vector
promoter-cloning vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--8.8; BglII--6.7, 2.8; EcoRI--7.2, 1.45, 0.57.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 7285 bp BamHI/HindIII fragment of YCp8 containing URA3 and CEN6 was ligated to a 1415 bp BamHI/HindIII fragment of YRp7-32cat (ATCC 37624).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

Shipped freeze-dried