YCp8cat (ATCC® 37633)

Applications: YC-type (centromeric) shuttle vectorpromoter-cloning vectorshuttle vector  /  Depositors: H Feldmann

Permits and Restrictions

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Designations YCp8cat
Depositors H Feldmann
Biosafety Level 1
Vector Information
Size (kb): 8.6999998092651370
Vector: YCp8cat (plasmid)
Promoters: Promoter none
Construction: YRp7-32cat (ATCC 37624), CEN6, URA3
Marker(s):TRP1,URA3,ampR
Construct size (kb): 8.699999809265137
Features: insert detection: CAT
marker(s): ampR, TRP1, URA3
promoter: none
replicon: pMB1, ARS1
centromere: CEN6
Applications
YC-type (centromeric) shuttle vector
promoter-cloning vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI/HindIII--8.8; BglII--6.7, 2.8; EcoRI--7.2, 1.45, 0.57.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 7285 bp BamHI/HindIII fragment of YCp8 containing URA3 and CEN6 was ligated to a 1415 bp BamHI/HindIII fragment of YRp7-32cat (ATCC 37624).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

Shipped freeze-dried
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