Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 6.6100001335144040
Vector: YIp5-32cat (plasmid)
Promoters: Promoter none
Construction: YIp5, YRp7-32cat (ATCC 37624)
Construct size (kb): 6.610000133514404
Features: insert detection: CAT
marker(s): ampR, URA3
YI-type (integrating) shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 0.64, 0.58; PstI--3.85, 3.20; PvuII--3.7, 3.25.
The depositor recommends using YRp7-17camp (ATCC 37630)
as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624
through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 5195 bp BamHI/HindIII fragment of YIp5 was ligated to a 1415 bp HindIII/BamHI fragment of YRp7-32cat (ATCC 37624)
Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257