YIp5-32cat (ATCC® 37628)

Applications: YI-type (integrating) shuttle vectorintegrating vectorpromoter-cloning vectorshuttle vector  /  Depositors: H Feldmann

Permits and Restrictions

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Designations YIp5-32cat
Depositors H Feldmann
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.6100001335144040
Vector: YIp5-32cat (plasmid)
Promoters: Promoter none
Construction: YIp5, YRp7-32cat (ATCC 37624)
Construct size (kb): 6.610000133514404
Features: insert detection: CAT
marker(s): ampR, URA3
promoter: none
replicon: pMB1
YI-type (integrating) shuttle vector
integrating vector
promoter-cloning vector
shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 0.64, 0.58; PstI--3.85, 3.20; PvuII--3.7, 3.25.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 5195 bp BamHI/HindIII fragment of YIp5 was ligated to a 1415 bp HindIII/BamHI fragment of YRp7-32cat (ATCC 37624).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

Shipped freeze-dried
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