YIp5-32cat (ATCC® 37628)

Applications: YI-type (integrating) shuttle vectorintegrating vectorpromoter-cloning vectorshuttle vector  /  Depositors: H Feldmann

Permits and Restrictions

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Designations YIp5-32cat
Depositors H Feldmann
Biosafety Level 1
Vector Information
Size (kb): 6.6100001335144040
Vector: YIp5-32cat (plasmid)
Promoters: Promoter none
Construction: YIp5, YRp7-32cat (ATCC 37624)
Marker(s):URA3,ampR
Construct size (kb): 6.610000133514404
Features: insert detection: CAT
marker(s): ampR, URA3
promoter: none
replicon: pMB1
Applications
YI-type (integrating) shuttle vector
integrating vector
promoter-cloning vector
shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--5.8, 0.64, 0.58; PstI--3.85, 3.20; PvuII--3.7, 3.25.
The depositor recommends using YRp7-17camp (ATCC 37630) as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624 through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 5195 bp BamHI/HindIII fragment of YIp5 was ligated to a 1415 bp HindIII/BamHI fragment of YRp7-32cat (ATCC 37624).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257

Shipped freeze-dried
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