Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Size (kb): 7.7600002288818360
Vector: YCp7-32cat (plasmid)
Promoters: Promoter none
Construction: YRp7-32cat (ATCC 37624)
Construct size (kb): 7.760000228881836
Features: insert detection: CAT
marker(s): ampR, TRP1
replicon: pMB1, ARS1
YC-type (centromeric) shuttle vector
Restriction digests of the clone give the following sizes (kb): EcoRI--6.2, 1.4, 0.56; PstI--6.5, 1.3; PvuII--6.0, 1.8.
The depositor recommends using YRp7-17camp (ATCC 37630)
as the reference for CAT expression and using the CAT assay methodology of Neumann, et al.
One of a series (ATCC 37624
through 37634) of Escherichia coli/Saccharomyces cerevisiae shuttle vectors for investigating control of expression in yeast.
A 1520 bp NdeI fragment containing CEN6 was cloned into the NdeI site of YRp7-32cat (ATCC 37624)
Mannhaupt G, et al. A series of shuttle vectors using chloramphenicol acetyltransferase as a reporter enzyme in yeast. Gene 67: 287-294, 1988. PubMed: 3049257