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Vector Information
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Size (kb): 4.5999999046325680 Vector: p305 (plasmid) Promoters: Promoter lac Construction: pUC18, aph, herpes simplex virus TK Marker(s):G418R,ampR Construct size (kb): 4.599999904632568 Features: insert detection: lacZ'
marker(s): ampR, G418R
promoter: lac
replicon: pMB1
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Applications
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shuttle vector vector permitting construction of fusion proteins
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Comments
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SmaI, HincII and EcoRI sites appear in both the polylinker and the aph cassette. Restriction digests of the clone give the following sizes (kb): HindIII--4.65; HincII--4.65; PstI--3.0, 1.7. An Escherichia coli/eukaryotic cell line shuttle vector containing lacZ and multiple cloning sites of pUC18 and the aph (neoR) gene under the control of herpes simplex virus tk promoter. A PvuII (position 628) of pUC18 was changed to BglII. The aph (aminoglycoside phosphotransferase) gene under the control of herpes simplex virus tk promoter was inserted at the BglII site. Aph is transcribed in the same direction as lacZ.
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References
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Lang JC, et al. pUC vectors capable of conferring neomycin resistance of eukaryotic cells. Nucleic Acids Res. 16: 7736, 1988. PubMed: 2842735
Dinakar Desai, personal communication
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