EMBL3-cos-Not (ATCC® 37603)

Applications: vector for constructing genomic libraries  /  Depositors: NE Murray

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Designations EMBL3-cos-Not
Depositors NE Murray
Biosafety Level 1
Vector Information
Size (kb): 48.2000007629394500
Vector: EMBL3-cos-Not (phage, lambda - replacement)
Construction: EMBL3cos, polylinker from EMBL301
Construct size (kb): 48.20000076293945
Features: replicon: lambda
Applications
vector for constructing genomic libraries
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--33.5, 14.5; BamHI--33.5, 14.5.
By labeling the left cohesive end, which is 200 bp from cloning sites, reliable restriction maps of clones can be made from partial digests.
For library construction, a double digest of the vector with BamHI + EcoRI and removal of the linker fragment reduces the number of background phage. Phosphatase treatment of digested vector eliminates background.
A modified EMBL vector, with all phage coding sequences to the right of the cloning sites to facilitate restriction mapping of clones. Can be used for genomic library construction.
References

Whittaker PA, et al. Enhanced recovery and restriction mapping of DNA fragments cloned in a new lambda vector. Nucleic Acids Res. 16: 6725-6736, 1988. PubMed: 2841642

Noreen E Murray, personal communication

Shipped freeze-dried
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