pSKS116 (ATCC® 37589)

Applications: shuttle vectorvector permitting construction of fusion proteins  /  Depositors: MJ Casadaban

Permits and Restrictions

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Designations pSKS116
Depositors MJ Casadaban
Biosafety Level 1
Vector Information
Size (kb): 3.7999999523162840
Vector: pSKS116 (plasmid)
Promoters: Promoter none
Construction: pBR322, herpes simplex virus TK
Marker(s):ampR,truncated TK
Construct size (kb): 3.799999952316284
Features: marker(s): ampR, truncated TK
promoter: none
replicon: pMB1
terminator: TK
enhancer: none
Applications
shuttle vector
vector permitting construction of fusion proteins
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--3.8; BamHI--3.8; HindIII--uncut; HincII--3.8.
This is a vector for the construction of thymidine kinase fusion proteins.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Shapira SK, Casadaban MJ. Hybrid protein thymidine kinase gene fusions: plasmid vectors for the study of transcription and translation initiation signals. Gene 52: 83-94, 1987. PubMed: 3036661

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet