pSKS116 (ATCC® 37589)

Applications: shuttle vectorvector permitting construction of fusion proteins  /  Depositors: MJ Casadaban

Permits and Restrictions

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Designations pSKS116
Depositors MJ Casadaban
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.7999999523162840
Vector: pSKS116 (plasmid)
Promoters: Promoter none
Construction: pBR322, herpes simplex virus TK
Marker(s):ampR,truncated TK
Construct size (kb): 3.799999952316284
Features: marker(s): ampR, truncated TK
promoter: none
replicon: pMB1
terminator: TK
enhancer: none
shuttle vector
vector permitting construction of fusion proteins
Restriction digests of the clone give the following sizes (kb): EcoRI--3.8; BamHI--3.8; HindIII--uncut; HincII--3.8.
This is a vector for the construction of thymidine kinase fusion proteins.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Shapira SK, Casadaban MJ. Hybrid protein thymidine kinase gene fusions: plasmid vectors for the study of transcription and translation initiation signals. Gene 52: 83-94, 1987. PubMed: 3036661

Shipped freeze-dried