pT109luc Plasmid in Escherichia coli (ATCC® 37583)

Applications:  expression vector  shuttle vector  /  Depositors: SK Nordeen

Permits and Restrictions

View Permits View Restrictions

Designations pT109luc Plasmid in Escherichia coli
Depositors SK Nordeen
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.09
Vector: pT109luc (plasmid)
Promoters: Promoter HSV TK
Construction: pXP2, herpes simplex virus TK promoter
Marker(s):ampR
Construct size (kb): 6.09
Features: marker(s): ampR
promoter: HSV TK
replicon: pMB1
terminator: none
enhancer: none
Applications
 expression vector
 shuttle vector
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; SalI--6.6.
There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts.
This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California.
One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells.
This contains bp -109 to +52 of the herpes simplex tk promoter; both GC + CCAAT are present.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Nordeen SK. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6: 454-458, 1988. PubMed: 2908509

de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727

Steven K Nordeen, personal communication

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Restrictions This product's use is governed by a Label License. For information on purchasing a license to use this product for purposes other than those permitted in the Label License, please contact Promega.
Basic Documentation