pT109luc Plasmid in Escherichia coli (ATCC® 37583)

Applications:  expression vector  shuttle vector  /  Depositors: SK Nordeen

Designations pT109luc Plasmid in Escherichia coli
Permits and Restrictions

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Depositors SK Nordeen
Biosafety Level 1
Vector Information
Size (kb): 6.09
Vector: pT109luc (plasmid)
Promoters: Promoter HSV TK
Construction: pXP2, herpes simplex virus TK promoter
Construct size (kb): 6.09
Features: marker(s): ampR
promoter: HSV TK
replicon: pMB1
terminator: none
enhancer: none
 expression vector
 shuttle vector
Restriction digests of the clone give the following sizes (kb): BamHI--6.6; HindIII--6.6; SalI--6.6.
There is cryptic promoter activity which may be more readily observed with weak promoters. Be careful to do all appropriate controls and map the transcripts.
This is a derivative of plasmids patented by the University of California and is not to be used for commercial purposes without prior arrangement with the University of California.
One of a series of luciferase vectors (ATCC 37574-37584) for assaying promoter activity in mammalian cells.
This contains bp -109 to +52 of the herpes simplex tk promoter; both GC + CCAAT are present.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Nordeen SK. Luciferase reporter gene vectors for analysis of promoters and enhancers. BioTechniques 6: 454-458, 1988. PubMed: 2908509

de Wet JR, et al. Firefly luciferease gene: Structure and expression in mammalian cells. Mol. Cell. Biol. 7: 725-737, 1987. PubMed: 3821727

Steven K Nordeen, personal communication

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
Restrictions This product's use is governed by a Label License. For information on purchasing a license to use this product for purposes other than those permitted in the Label License, please contact Promega.
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