pcos1EMBL (ATCC® 37568)

Applications: vector for constructing cDNA libraries  /  Depositors: A Craig

Permits and Restrictions

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Designations pcos1EMBL
Depositors A Craig
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 6.3000001907348630
Vector: pcos1EMBL (cosmid)
Promoters: Promoter none
Construction: R6K, pcos4EMBL
Marker(s):kanR,tetR
Construct size (kb): 6.300000190734863
Features: marker(s): kanR, tetR
promoter: none
replicon: R6K
terminator: none
enhancer: none
Applications
vector for constructing cDNA libraries
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.3; SalI--6.3.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
One of a family of cosmid vectors (ATCC 37568-37571) containing a R6K origin.
Contains one lambda cos site.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 30.0°C
References

Ehrich E, et al. A family of cosmid vectors with the multi-copy R6K replication origin. Gene 57: 229-237, 1987. PubMed: 2961651

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet