pcos1EMBL (ATCC® 37568)

Applications: vector for constructing cDNA libraries  /  Depositors: A Craig

Permits and Restrictions

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Designations pcos1EMBL
Depositors A Craig
Biosafety Level 1
Vector Information
Size (kb): 6.3000001907348630
Vector: pcos1EMBL (cosmid)
Promoters: Promoter none
Construction: R6K, pcos4EMBL
Marker(s):kanR,tetR
Construct size (kb): 6.300000190734863
Features: marker(s): kanR, tetR
promoter: none
replicon: R6K
terminator: none
enhancer: none
Applications
vector for constructing cDNA libraries
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--6.3; SalI--6.3.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
One of a family of cosmid vectors (ATCC 37568-37571) containing a R6K origin.
Contains one lambda cos site.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 30.0°C
References

Ehrich E, et al. A family of cosmid vectors with the multi-copy R6K replication origin. Gene 57: 229-237, 1987. PubMed: 2961651

Shipped freeze-dried
Product Sheet
Product Sheet
Product Sheet