piAN13 (ATCC® 37544)

Applications: vector for other uses  /  Depositors: HV Huang

Designations piAN13
Depositors HV Huang
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 0.8999999761581421
Vector: piAN13 (plasmid)
Promoters: Promoter none
Construction: piAN7 and M13mp11
Marker(s):ampR,kanR,tetR
Construct size (kb): 0.8999999761581421
Features: marker(s): ampR, kanR, tetR
promoter: none
replicon: pMB1
Applications
vector for other uses
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--0.88; HindIII--0.88; EcoRI--0.88.
Designed for screening DNA libraries by homologous recombination using Syrinx2A bacteriophage vector.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Lutz CT, et al. Syrinx 2A: An improved lambda phage vector designed for screening DNA libraries by recombination in vivo. Proc. Natl. Acad. Sci. USA 84: 4379-4383, 1987. PubMed: 2955406

Shipped freeze-dried
Shipping Information Distributed: freeze-dried