pJRD215 (ATCC® 37533)

Applications: shuttle vectorvector for constructing genomic libraries  /  Depositors: J Davison

Permits and Restrictions

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Designations pJRD215
Depositors J Davison
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 10.1999998092651400
Vector: pJRD215 (cosmid)
Promoters: Promoter none
Construction: RSF1010, lambda cos, Tn5, pBR322, pIC20R
Marker(s):kanR,strR
Construct size (kb): 10.19999980926514
Features: marker(s): kanR, strR
promoter: none
replicon: RSF1010 (IncQ)
terminator: none
enhancer: none
Applications
shuttle vector
vector for constructing genomic libraries
Comments
Broad host range, multiple cloning site cosmid that can be transferred by conjugation.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
References

Davison J, et al. Vectors with restriction site banks V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites. Gene 51: 275-280, 1987. PubMed: 3036654

Shipped frozen
Product Sheet
Product Sheet
Product Sheet