pJRD215 (ATCC® 37533)

Applications: shuttle vectorvector for constructing genomic libraries  /  Depositors: J Davison

Permits and Restrictions

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Designations pJRD215
Depositors J Davison
Biosafety Level 1
Vector Information
Size (kb): 10.1999998092651400
Vector: pJRD215 (cosmid)
Promoters: Promoter none
Construction: RSF1010, lambda cos, Tn5, pBR322, pIC20R
Marker(s):kanR,strR
Construct size (kb): 10.19999980926514
Features: marker(s): kanR, strR
promoter: none
replicon: RSF1010 (IncQ)
terminator: none
enhancer: none
Applications
shuttle vector
vector for constructing genomic libraries
Comments
Broad host range, multiple cloning site cosmid that can be transferred by conjugation.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
References

Davison J, et al. Vectors with restriction site banks V. pJRD215, a wide-host-range cosmid vector with multiple cloning sites. Gene 51: 275-280, 1987. PubMed: 3036654

Shipped frozen
Product Sheet
Product Sheet
Product Sheet