LORIST2 (ATCC® 37531)

Applications: vector for constructing genomic librariesvector for other usesvector permitting RNA synthesis in vitro  /  Depositors: TJ Gibson

Permits and Restrictions

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Designations LORIST2
Depositors TJ Gibson
Biosafety Level 1
Vector Information
Size (kb): 5.5599999427795410
Vector: LORIST2 (cosmid)
Promoters: Promoter T7
Marker(s):kanR,neoR
Construct size (kb): 5.559999942779541
Features: marker(s): kanR, neoR
promoter: SP6, T7
replicon: lambda
terminator: trpA rrnC
Applications
vector for constructing genomic libraries
vector for other uses
vector permitting RNA synthesis in vitro
Comments
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; HindIII--5.8.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Cosmid vector for genomic library construction. Insert-end-specific probes may be generated from T7 and SP6 promoters.
Transcription terminators somewhat insulate the vector genes from insert transcription.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
References

Gibson TJ, et al. Lorist2, a cosmid with transcriptional terminators insulating vector genes from interference by promoters within the insert: effect on DNA yield and cloned insert frequency. Gene 53: 275-281, 1987. PubMed: 3301536

Shipped freeze-dried
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