LORIST2 (ATCC® 37531)

Applications: vector for constructing genomic librariesvector for other usesvector permitting RNA synthesis in vitro  /  Depositors: TJ Gibson

Permits and Restrictions

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Designations LORIST2
Depositors TJ Gibson
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.5599999427795410
Vector: LORIST2 (cosmid)
Promoters: Promoter T7
Construct size (kb): 5.559999942779541
Features: marker(s): kanR, neoR
promoter: SP6, T7
replicon: lambda
terminator: trpA rrnC
vector for constructing genomic libraries
vector for other uses
vector permitting RNA synthesis in vitro
Restriction digests of the clone give the following sizes (kb): BamHI--5.6; HindIII--5.8.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
Cosmid vector for genomic library construction. Insert-end-specific probes may be generated from T7 and SP6 promoters.
Transcription terminators somewhat insulate the vector genes from insert transcription.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C

Gibson TJ, et al. Lorist2, a cosmid with transcriptional terminators insulating vector genes from interference by promoters within the insert: effect on DNA yield and cloned insert frequency. Gene 53: 275-281, 1987. PubMed: 3301536

Shipped freeze-dried
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