pBR328 (ATCC® 37517)

Depositors: EM Lederberg

Permits and Restrictions

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Designations pBR328
Depositors EM Lederberg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: E. coli RR1
Vector Information
Size (kb): 4.9000000953674320
Vector: pBR328 (plasmid)
Promoters: Promoter none
Construction: pBR327 ori, pBR325 cml
Marker(s):ampR,cmlR,tetR
Construct size (kb): 4.900000095367432
Features: marker(s): ampR, cmlR, tetR
promoter: none
replicon: pMB1
terminator: none
Comments
A general purpose plasmid vector.
Contains a 482 bp inverted duplication. Constructed from the PstI/BamHI fragment of pBR327 containing the replication origin and the PstI/BamHI fragment of pBR325 containing the cml gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Soberon X, et al. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene 9: 287-305, 1980. PubMed: 6248430

Balbas P, et al. Plasmid vector pBR322 and its special-purpose derivatives--a review. Gene 50: 3-40, 1986. PubMed: 3034735

Shipped frozen
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid.