pBR328 (ATCC® 37517)

Depositors: EM Lederberg

Designations pBR328
Permits and Restrictions

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Depositors EM Lederberg
Disclosure REPOS
Biosafety Level 1
Host
Distribution host: E. coli RR1
Vector Information
Size (kb): 4.9000000953674320
Vector: pBR328 (plasmid)
Promoters: Promoter none
Construction: pBR327 ori, pBR325 cml
Marker(s):ampR,cmlR,tetR
Construct size (kb): 4.900000095367432
Features: marker(s): ampR, cmlR, tetR
promoter: none
replicon: pMB1
terminator: none
Comments
A general purpose plasmid vector.
Contains a 482 bp inverted duplication. Constructed from the PstI/BamHI fragment of pBR327 containing the replication origin and the PstI/BamHI fragment of pBR325 containing the cml gene.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Soberon X, et al. Construction and characterization of new cloning vehicles. IV. Deletion derivatives of pBR322 and pBR325. Gene 9: 287-305, 1980. PubMed: 6248430

Balbas P, et al. Plasmid vector pBR322 and its special-purpose derivatives--a review. Gene 50: 3-40, 1986. PubMed: 3034735

Shipped frozen
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid.