pBR327 (ATCC® 37516)

Depositors: EM Lederberg

Designations pBR327
Permits and Restrictions

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Depositors EM Lederberg
Biosafety Level 1
Vector Information
Size (kb): 3.2730000019073490
Vector: pBR327 (plasmid)
Construct size (kb): 3.273000001907349
Features: marker(s): ampR, tetR
replicon: pMB1
A general purpose plasmid vector.
Derived from pBR322 by deleting sequences between nt 1430 (AvaI) and 2519, thus removing the bom or Mob site.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Recomb. DNA Tech. Bull. 2: 1980..

Twigg AJ, Sherratt D. Trans-complementable copy-number mutants of plasmid ColE1. Nature 283: 216-218, 1980. PubMed: 7350544

Covarrubias L, et al. Construction and characaterization of new cloning vehicles V. mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328. Gene 13: 25-35, 1981. PubMed: 6263753

Balbas P, et al. Plasmid vector pBR322 and its special-purpose derivatives--a review. Gene 50: 3-40, 1986. PubMed: 3034735

Shipped freeze-dried