pVA517A THROUGH H (ATCC® 37514)

Other IDs:

Nucleotide (GenBank) : L04669 Saccharomyces cerevisiae (clone pSMD1-PRP38) D1 snRNP (SMD1) gene,

 /  Applications: May be used as covalently-closed circular markers in agarose gel electrophoresis.  /  Depositors: EM Lederberg

Permits and Restrictions

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Designations pVA517A THROUGH H
Other IDs

Nucleotide (GenBank) : L04669 Saccharomyces cerevisiae (clone pSMD1-PRP38) D1 snRNP (SMD1) gene,

Depositors EM Lederberg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 0E-16
Vector: pVA517A THROUGH H (plasmid)
Construction: natural isolate
Construct size (kb): 0.0
Applications
May be used as covalently-closed circular markers in agarose gel electrophoresis.
Comments
Isolate plasmids from mid-log phase cells following the protocol in Biochemistry 9: 4428-4439, 1970 exactly. DNA preparations are best stored at -20C divided into small samples.
natural isolate
May be used as covalently-closed circular markers in agarose gel electrophoresis. This strain contains 8 plasmids: A--53.23 kb, B--7.16 kb, C--5.45 kb, D--5.04 kb, E--3.91 kb, F--3.02 kb, G--2.66 kb, H--2.02 kb.
Media ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37.0°C
References

Macrina FL, et al. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1: 417-420, 1978. PubMed: 372973

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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