pVA517A THROUGH H (ATCC® 37514)

Other IDs:

Nucleotide (GenBank) : L04669 Saccharomyces cerevisiae (clone pSMD1-PRP38) D1 snRNP (SMD1) gene,

 /  Applications: May be used as covalently-closed circular markers in agarose gel electrophoresis.  /  Depositors: EM Lederberg

Permits and Restrictions

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Designations pVA517A THROUGH H
Depositors EM Lederberg
Other IDs

Nucleotide (GenBank) : L04669 Saccharomyces cerevisiae (clone pSMD1-PRP38) D1 snRNP (SMD1) gene,

Biosafety Level 1
Vector Information
Size (kb): 0E-16
Vector: pVA517A THROUGH H (plasmid)
Construction: natural isolate
Construct size (kb): 0.0
Applications
May be used as covalently-closed circular markers in agarose gel electrophoresis.
Comments
Isolate plasmids from mid-log phase cells following the protocol in Biochemistry 9: 4428-4439, 1970 exactly. DNA preparations are best stored at -20C divided into small samples.
natural isolate
May be used as covalently-closed circular markers in agarose gel electrophoresis. This strain contains 8 plasmids: A--53.23 kb, B--7.16 kb, C--5.45 kb, D--5.04 kb, E--3.91 kb, F--3.02 kb, G--2.66 kb, H--2.02 kb.
Media ATCC® Medium 3: Nutrient agar or nutrient broth
Growth Conditions
Temperature: 37.0°C
References

Macrina FL, et al. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid 1: 417-420, 1978. PubMed: 372973

Shipped freeze-dried
Shipping Information Distributed: freeze-dried
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