pSRW25 (ATCC® 37506)

Applications: expression vector  /  Depositors: CM Thomas

Designations pSRW25
Permits and Restrictions

View Permits

Depositors CM Thomas
Biosafety Level 1
Vector Information
Size (kb): 12.6000003814697300
Vector: pSRW25 (plasmid)
Promoters: Promoter trp
Construction: pRK2501, EcoRI-SphI trpR from pSRW21
Construct size (kb): 12.60000038146973
Features: marker(s): kanR, tetR
promoter: trp
replicon: pMB1, RK2
expression vector
Plasmid can replicate in most gram-negative bacteria except Bacteroides.
Constructed to provide tight but inducible regulation from the trp promoter on a high copy number plasmid.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C

Warne SR, et al. Use of a modified Escherichia coli trpR gene to obtain tight regulation of high-copy-number expression vectors. Gene 46: 103-112, 1986. PubMed: 3026921

Christopher M Thomas, personal communication

Shipped freeze-dried