Size (kb): 12.6000003814697300
Vector: pSRW25 (plasmid)
Promoters: Promoter trp
Construction: pRK2501, EcoRI-SphI trpR from pSRW21
Construct size (kb): 12.60000038146973
Features: marker(s): kanR, tetR
replicon: pMB1, RK2
Plasmid can replicate in most gram-negative bacteria except Bacteroides.
Constructed to provide tight but inducible regulation from the trp promoter on a high copy number plasmid.
Warne SR, et al. Use of a modified Escherichia coli trpR gene to obtain tight regulation of high-copy-number expression vectors. Gene 46: 103-112, 1986. PubMed: 3026921
Christopher M Thomas, personal communication