F'lacZ+cI857 (ATCC® 37446)

Applications: expression vector  /  Depositors: M Mieschendahl

Designations F'lacZ+cI857
Permits and Restrictions

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Depositors M Mieschendahl
Biosafety Level 1
Vector Information
Size (kb): 0E-16
Vector: F'lacZ+cI857 (plasmid)
Promoters: Promoter lambda PL
Construct size (kb): 0.0
Features: insert detection: lacZ'
marker(s): nalR
promoter: lambda PL, lambda PR
replicon: F
repressor gene: cI857
enhancer: none
expression vector
Facilitates construction and regulation of lambda promoter-dependent expression systems without using defective prophages.
Media ATCC® Medium 1065: LB Agar/Broth, Miller
Growth Conditions
Temperature: 30.0°C

Mieschendahl M, Muller-Hill B. F'-coded, temperature-sensitive alpha cI857 repressor gene for easy construction and regulation of alpha promoter-dependent expression systems. J. Bacteriol. 164: 1366-1369, 1985. PubMed: 2999084

Shipped freeze-dried