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Vector Information
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Size (kb): 8.1000003814697270 Vector: pEMBLYe31 (phagemid) Construction: pEMBL9, ( ATCC 37395), bacteriophage f1, fragment of CV13 Marker(s):LEU2,ampR Construct size (kb): 8.100000381469727 Features: insert detection: lacZ'
marker(s): ampR, LEU2
replicon: pMB1, f1, 2 micron
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Applications
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YE-type (episomal) shuttle vector shuttle vector vector permitting construction of fusion proteins vector permitting production of single-stranded DNA
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Comments
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ssDNA-producing plasmid with polylinker in lacZ' and marker selectable in yeast.
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References
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Dente L, et al. pEMBL: a new family of single stranded plasmids. Nucleic Acids Res. 11: 1645-1655, 1983. PubMed: 6300771
Baldari C, Cesareni G. Plasmids pEMBLY:new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences. Gene 35: 27-32, 1985. PubMed: 3896935
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