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pEMBLYe31 (ATCC® 37405™)

Applications: YE-type (episomal) shuttle vectorshuttle vectorvector permitting construction of fusion proteinsvector permitting production of single-stranded DNA  /  Depositors: G Cesareni

Designations pEMBLYe31
Permits and Restrictions

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Depositors G Cesareni
Biosafety Level 1
Vector Information
Size (kb): 8.1000003814697270
Vector: pEMBLYe31 (phagemid)
Construction: pEMBL9, (ATCC 37395), bacteriophage f1, fragment of CV13
Marker(s):LEU2,ampR
Construct size (kb): 8.100000381469727
Features: insert detection: lacZ'
marker(s): ampR, LEU2
replicon: pMB1, f1, 2 micron
Applications
YE-type (episomal) shuttle vector
shuttle vector
vector permitting construction of fusion proteins
vector permitting production of single-stranded DNA
Comments
ssDNA-producing plasmid with polylinker in lacZ' and marker selectable in yeast.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Dente L, et al. pEMBL: a new family of single stranded plasmids. Nucleic Acids Res. 11: 1645-1655, 1983. PubMed: 6300771

Baldari C, Cesareni G. Plasmids pEMBLY:new single-stranded shuttle vectors for the recovery and analysis of yeast DNA sequences. Gene 35: 27-32, 1985. PubMed: 3896935

Shipped freeze-dried