pSS25 (ATCC® 37392)

Applications: vector for constructing cDNA librariesvector permitting production of single-stranded DNA  /  Depositors: J Kowalski

Permits and Restrictions

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Designations pSS25
Depositors J Kowalski
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.5999999046325680
Vector: pSS25 (phagemid)
Construction: pSS34
Marker(s):ampR
Construct size (kb): 3.599999904632568
Features: marker(s): ampR
replicon: pMB1, f1
terminator: none
Applications
vector for constructing cDNA libraries
vector permitting production of single-stranded DNA
Comments
Plasmid vector for construction of cDNA libraries.
The f1 intergenic region for packaging of ssDNA was inserted into an Okayama & Berg vector. SV40 sequences have been removed.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Kowalski J, et al. Vectors for the direct selection of cDNA clones corresponding to mammalian cell mRNA of low abundance. Gene 35: 45-54, 1985. PubMed: 2863196

Shipped freeze-dried
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