lambdaSE4 (ATCC® 37387)

Applications: vector permitting positive selection for insertsvector with low copy number  /  Depositors: SJ Elledge

Designations lambdaSE4
Permits and Restrictions

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Depositors SJ Elledge
Biosafety Level 1
Vector Information
Size (kb): 46.0000000000000000
Vector: lambdaSE4 (phagemid)
Construction: lambda1059, pDPT427
Construct size (kb): 46.0
Features: insert detection: Spi+
marker(s): ampR, spcR, strR
replicon: pMB1, NR1, lambda
repressor gene: cI857
vector permitting positive selection for inserts
vector with low copy number
Plasmid is temperature inducible to produce infectious phage particles.
This phagemid grows lytically as phage in non-immune hosts or lysogenically as a phagemid in an immune host.
A phagemid vector useful for identification of genes by complementation.
With a BamHI digest, the left arm is 24.1 kb, the stuffer fragment encoding the Spi+ phenotype is 16 kb, and the right arm is 8.6 kb.
This vector uses Spi- selection for insertion of DNA into the vector and has the ability to accept 2 - 19 kb Sau3AI, BamHI, BglII, BclI, or XhoII fragments.
This vector was constructed by joining the 4.7 kb BamHI-BglII fragment of pDPT427 (low copy number replicon + spectinomycin resistance) to the left arm of lambda1059.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C

Elledge SJ, Walker GC. Phasmid vectors for identification of genes by complementation of Escherichia coli mutants. J. Bacteriol. 162: 777-783, 1985. PubMed: 2985547

Shipped freeze-dried