pfdB2 (ATCC® 37371)

Applications: vector permitting production of single-stranded DNA  /  Depositors: K Geider

Designations pfdB2
Permits and Restrictions

View Permits

Depositors K Geider
Biosafety Level 1
Vector Information
Size (kb): 1.8899999856948850
Vector: pfdB2 (plasmid)
Promoters: Promoter none
Construction: pfdA2, pIG-414RB
Construct size (kb): 1.889999985694885
Features: marker(s): kanR
promoter: none
replicon: fd
terminator: none
enhancer: none
vector permitting production of single-stranded DNA
The plasmid is lost at 42C if grown without antibiotic. The host Escherichia coli strain must express gene 2 of filamentous bacteriophages.
Vector with bacteriophage fd replication system.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 37.0°C

Geider K, et al. A plasmid cloning system utilizing replication and packaging functions of the filamentous bacteriophage fd. Gene 33: 341-349, 1985. PubMed: 2989095

Shipped freeze-dried