pRB273 (ATCC® 37340)

Applications: shuttle vector  /  Depositors: R Bruckner

Permits and Restrictions

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Designations pRB273
Depositors R Bruckner
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.5999999046325680
Vector: pRB273 (plasmid)
Construction: pBR322, pRB103
Marker(s):ampR,kanR
Construct size (kb): 5.599999904632568
Features: marker(s): ampR, kanR
replicon: pMB1, pUB110
enhancer: none
Applications
shuttle vector
Comments
A shuttle vector which confers both ampicillin and kanamycin resistance in Escherichia coli but only kanamycin resistance in Bacillus subtilis.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Bruckner R, et al. Expression of a chloramphenicol-resistance determinant carried on hybrid plasmids in Gram-positive and Gram-negative bacteria. Gene 32: 151-160, 1984. PubMed: 6442250

Shipped freeze-dried