pLA7 (ATCC® 37281)

Applications: vector permitting positive selection for inserts  /  Depositors: IR Beacham

Permits and Restrictions

View Permits

Designations pLA7
Depositors IR Beacham
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 4.0999999046325680
Vector: pLA7 (plasmid)
Construction: pCB1, pBR322
Construct size (kb): 4.099999904632568
Features: insert detection: ush+
marker(s): ampR, ush
replicon: pMB1
vector permitting positive selection for inserts
Propagation in a dam- host is necessary for cleavage at the BclI site.
A positive selection vector for cloning Sau3AI-generated DNA fragments.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Burns DM, Beacham IR. Positive selector vectors: a small plasmid vector useful for the direct selection of Sau3A-generated overlapping DNA fragments. Gene 27: 323-325, 1984. PubMed: 6329908

Shipped freeze-dried