pMMB34 [JD51] (ATCC® 37249)

Applications: shuttle vectorvector for constructing genomic libraries  /  Depositors: J Frey

Permits and Restrictions

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Designations pMMB34 [JD51]
Depositors J Frey
Biosafety Level 1
Vector Information
Size (kb): 13.75
Vector: pMMB34 (cosmid)
Construction: RSF1010, pHC79
Marker(s):kanR
Construct size (kb): 13.75
Features: marker(s): kanR
replicon: R300B
Applications
shuttle vector
vector for constructing genomic libraries
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A broad host range cosmid vector.
It was constructed by replacing the 0.75 kb PstI fragment of pMMB7 by the 5.7 kb PstI fragment of pMMB31, which contains the lambda-cos site and the Km-resistance gene. It differs from pMMB33, ATCC# 37248, only by the orientation of the 5.7 kb fragment.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
References

Frey J, et al. Stable cosmid vectors that enable the introduction of cloned fragments into a wide range of Gram-negative bacteria. Gene 24: 299-308, 1983. PubMed: 6315541

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the cosmid

Product Sheet
Product Sheet
Product Sheet