pMMB34 [JD51] (ATCC® 37249)

Applications: shuttle vectorvector for constructing genomic libraries  /  Depositors: J Frey

Designations pMMB34 [JD51]
Permits and Restrictions

View Permits

Depositors J Frey
Biosafety Level 1
Vector Information
Size (kb): 13.75
Vector: pMMB34 (cosmid)
Construction: RSF1010, pHC79
Construct size (kb): 13.75
Features: marker(s): kanR
replicon: R300B
shuttle vector
vector for constructing genomic libraries
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A broad host range cosmid vector.
It was constructed by replacing the 0.75 kb PstI fragment of pMMB7 by the 5.7 kb PstI fragment of pMMB31, which contains the lambda-cos site and the Km-resistance gene. It differs from pMMB33, ATCC# 37248, only by the orientation of the 5.7 kb fragment.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the cosmid