pMMB34 [JD51] (ATCC® 37249)

Applications: shuttle vectorvector for constructing genomic libraries  /  Depositors: J Frey

Permits and Restrictions

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Designations pMMB34 [JD51]
Depositors J Frey
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 13.75
Vector: pMMB34 (cosmid)
Construction: RSF1010, pHC79
Marker(s):kanR
Construct size (kb): 13.75
Features: marker(s): kanR
replicon: R300B
Applications
shuttle vector
vector for constructing genomic libraries
Comments
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A broad host range cosmid vector.
It was constructed by replacing the 0.75 kb PstI fragment of pMMB7 by the 5.7 kb PstI fragment of pMMB31, which contains the lambda-cos site and the Km-resistance gene. It differs from pMMB33, ATCC# 37248, only by the orientation of the 5.7 kb fragment.
Media ATCC® Medium 1236: LB Medium (ATCC medium 1065) with 25 mcg/ml kanamycin
Growth Conditions
Temperature: 30.0°C
References

Frey J, et al. Stable cosmid vectors that enable the introduction of cloned fragments into a wide range of Gram-negative bacteria. Gene 24: 299-308, 1983. PubMed: 6315541

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the cosmid

Product Sheet
Product Sheet
Product Sheet