Carnegie 20 (ATCC® 37242)

Applications: integrating vectorshuttle vector  /  Depositors: GM Rubin

Permits and Restrictions

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Designations Carnegie 20
Depositors GM Rubin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 10.8000001907348600
Vector: Carnegie 20 (plasmid)
Construction: pUC8, Drosophila melanogaster
Construct size (kb): 10.80000019073486
Features: marker(s): ampR
replicon: pMB1
integrating vector
shuttle vector
This is a plasmid vector containing a non-autonomous P element from Drosophila melanogaster with a 7.2 kb HindIII insert containing the rosy gene. It should be used with a helper plasmid such as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Spradling AC, Rubin GM. Transposition of cloned P elements into Drosophila germ line chromosomes. Science 218: 341-347, 1982. PubMed: 6289435

Rubin GM, Spradling AC. Vectors for P element-mediated gene transfer in Drosophila. Nucleic Acids Res. 11: 6341-6351, 1983. PubMed: 6312420

Shipped freeze-dried