Carnegie 20 (ATCC® 37242)

Applications: integrating vectorshuttle vector  /  Depositors: GM Rubin

Designations Carnegie 20
Permits and Restrictions

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Depositors GM Rubin
Biosafety Level 1
Vector Information
Size (kb): 10.8000001907348600
Vector: Carnegie 20 (plasmid)
Construction: pUC8, Drosophila melanogaster
Construct size (kb): 10.80000019073486
Features: marker(s): ampR
replicon: pMB1
integrating vector
shuttle vector
This is a plasmid vector containing a non-autonomous P element from Drosophila melanogaster with a 7.2 kb HindIII insert containing the rosy gene. It should be used with a helper plasmid such as ppi25.1 (ATCC 37236) or ppi25.7 (ATCC 37237).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Spradling AC, Rubin GM. Transposition of cloned P elements into Drosophila germ line chromosomes. Science 218: 341-347, 1982. PubMed: 6289435

Rubin GM, Spradling AC. Vectors for P element-mediated gene transfer in Drosophila. Nucleic Acids Res. 11: 6341-6351, 1983. PubMed: 6312420

Shipped freeze-dried