pRSVgpt (ATCC® 37199)

Other IDs:

Genbank : M83240 Genbank sequence

 /  Applications: shuttle vector  /  Depositors: BH Howard

Permits and Restrictions

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Designations pRSVgpt
Depositors BH Howard
Other IDs

Genbank : M83240 Genbank sequence

Biosafety Level 1
Vector Information
Size (kb): 0E-16
Vector: pRSVgpt (plasmid)
Promoters: Promoter RSV LTR
Construction: pRSVcat, pSV2gpt
Marker(s):ampR,xgpt
Construct size (kb): 3.557
Features: marker(s): ampR, xgpt
promoter: RSV LTR
replicon: pMB1
Applications
shuttle vector
Comments
Escherichia coli/mammalian cell culture vector with selectable markers in both systems
Plasmid vector containing the Escherichia coli xanthine guanine phosphoribosyltransferase (gpt) gene as a selection marker and the RSV LTR promoter.
Plasmid has been shown to stably transfect several mammalian cell lines (Science 221: 551-553, 1983).
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
References

Gorman C, et al. High efficiency DNA-mediated transformation of primate cells. Science 221: 551-553, 1983. PubMed: 6306768

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid

Product Sheet
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