pY2 (ATCC® 37186)

Applications: expression vector  /  Depositors: JJ Rossi

Designations pY2
Permits and Restrictions

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Depositors JJ Rossi
Biosafety Level 1
Vector Information
Size (kb): 4.6999998092651370
Vector: pY2 (plasmid)
Promoters: Promoter synthetic
Construction: pBR327
Construct size (kb): 4.699999809265137
Features: marker(s): ampR, cmlR, tetR
promoter: synthetic
replicon: pMB1
expression vector
Contains a synthetic consensus Escherichia coli RNA polymerase promoter, HindIII CAT gene cartridge, and pBR327 amp and tet resistance genes.
Identical to pXJ002 (ATCC 37183) except the transcription start site is altered from CCC to CAT.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Rossi JJ, et al. Biological expression of an Escherichia coli sonsesus sequence promoter and some mutant derivatives. Proc. Natl. Acad. Sci. USA 80: 3203-3207, 1983. PubMed: 6304698

Shipped freeze-dried