pSV3-dhfr (ATCC® 37147)

Applications: expression vectorshuttle vector  /  Depositors: P Berg

Designations pSV3-dhfr
Depositors P Berg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 8.4
Vector: pSV3-dhfr (plasmid)
Promoters: Promoter SV40
Construction: pBR322, SV40, mouse
Construct size (kb): 8.4
Features: marker(s): ampR, mtxR
other: SV40 large T antigen
promoter: SV40
replicon: pMB1
expression vector
shuttle vector
Confers selective advantage to mammalian cell lines grown in the presence of methotrexate. Selectable marker in dhfr- cell culture cells.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Subramani S, et al. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell. Biol. 1: 854-864, 1981. PubMed: 9279398

Shipped freeze-dried
Shipping Information

Shipped:  Freeze dried E. coli containing the plasmid.