pSV3-dhfr plasmid in Escherichia coli (ATCC® 37147)

Applications: Expression vectorShuttle vector  /  Depositors: P Berg, Stanford University Medical Center

Designations pSV3-dhfr plasmid in Escherichia coli
Depositors P Berg, Stanford University Medical Center
Biosafety Level 1
Host Escherichia coli HB101
Vector Information
Construct size (kb): 8.4
Vector: pSV3-dhfr (plasmid)
Construction: pBR322, SV40, mouse

Features:
marker(s):
ampR, mtxR
other: SV40 large T antigen
promoter: SV40
replicon: pMB1
Applications
Expression vector
Shuttle vector
Comments
Confers selective advantage to mammalian cell lines grown in the presence of methotrexate. Selectable marker in dhfr- cell culture cells.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37°C
References

Subramani S, et al. Expression of the mouse dihydrofolate reductase complementary deoxyribonucleic acid in simian virus 40 vectors. Mol. Cell. Biol. 1: 854-864, 1981. PubMed: 9279398

Shipped freeze-dried
Shipping Information Plasmid in E. coli