pKO11 (ATCC® 37122)

Applications: promoter-cloning vector  /  Depositors: M Rosenberg

Permits and Restrictions

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Designations pKO11
Depositors M Rosenberg
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 3.9000000953674320
Vector: pKO11 (plasmid)
Promoters: Promoter none
Construction: pKO1 (ATCC 37126)
Construct size (kb): 3.900000095367432
Features: insert detection: galK
marker(s): ampR
promoter: none
replicon: pMB1
promoter-cloning vector
This is a promoter cloning vector using expression of the promoterless galK gene for positive or color (MacConkey galactose indicator plates) selection.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

McKenney K, et al. A system to study promoter and terminator signals recognized by Escherichia coli RNA polymerase. Gene Amplif. Anal. 2: 383-415, 1981. PubMed: 6101056

Adams CW, Hatfield GW. Effects of promoter strengths and growth conditions on copy number of transcriptions-fusion vectors. J. Biol. Chem. 259: 7399-7403, 1984. PubMed: 6736011

Nierman WCVectors for cloning promoters and terminatorsIn: Nierman WCVectors: a survey of molecular cloning vectors and their usesBostonButterworthpp. 153-177, 1988

Shipped freeze-dried