pS194 (ATCC® 37082)

Applications: shuttle vector  /  Depositors: L Philipson

Designations pS194
Permits and Restrictions

View Permits

Depositors L Philipson
Biosafety Level 2
Vector Information
Size (kb): 4.3969998359680180
Vector: pS194 (plasmid)
Construction: natural isolate
Construct size (kb): 4.396999835968018
Features: marker(s): strR
replicon: pS174
shuttle vector
Contains one EcoRI and one HindIII site and no sites for BamHI or SmaI.
natural isolate
Vector for construction and propagation of hybrid DNA in Staphylococcus aureus or Bacillus subtilis.
Media ATCC Medium 1065 (see below) plus streptomycin ATCC Medium 1065: Tryptone (Difco 0123), 10.0 g Yeast Extract (Difco 0127), 5.0 g NaCl, 10.0 g Distilled water, 1.0 L
Growth Conditions
Temperature: 30.0°C

Lofdahl S, et al. Characterization of small plasmids from Staphylococcus aureus. Gene 3: 145-149, 1978. PubMed: 95989

Projan SJ, et al. Nucleotide sequence of pS194, a streptomycin-resistance plasmid from Staphylococcus aureus. Nucleic Acids Res. 16: 2179-2187, 1988. PubMed: 3357770

Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of Staphylococcus aureus containing the plasmid.