pJB8 (ATCC® 37074)

Applications: vector for constructing genomic libraries  /  Depositors: D Ish-Horowicz, JF Burke

Designations pJB8
Permits and Restrictions

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Depositors D Ish-Horowicz, JF Burke
Biosafety Level 1
Vector Information
Size (kb): 5.4000000953674320
Vector: pJB8 (cosmid)
Construction: HomerI
Construct size (kb): 5.400000095367432
Features: marker(s): ampR
replicon: pMB1
vector for constructing genomic libraries
Restriction digests analyzed on agarose gels give the following sizes (kb): HindIII--5.4; BamHI--5.4; PstI/HindIII--4.6, 0.8; PstI/SalI--3.3, 2.3.
IMPORTANT: To prevent amplification of a rearranged and/or deleted cosmid, we recommend streaking on LB + amp plates at 30C and picking small colonies for liquid culture.
A cosmid vector containing a single cos site.
Cloning into the BamHI or EcoRI site involves 2 preparations of dephosphorylated vector, one linearized with HindIII, the other linearized with SalI.
An equimolar ratio of right-hand and left-hand cos containing vector fragments, digested with BamHI or EcoRI can be ligated to appropriately digested and dephosphorylated inserts.
Only molecules with 2 cos sites separated by 37-50 kb can be packaged.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 30.0°C

Ish-Horowicz D, Burke JF. Rapid and efficient cosmid cloning. Nucleic Acids Res. 9: 2989-2998, 1981. PubMed: 6269067

Shipped freeze-dried