YIp5 [pRB12] (ATCC® 37061)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: D Botstein

Designations YIp5 [pRB12]
Permits and Restrictions

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Depositors D Botstein
Biosafety Level 1
Vector Information
Size (kb): 5.5409998893737790
Vector: YIp5 (plasmid)
Construction: pBR322, lambda590-Sc2904
Construct size (kb): 5.540999889373779
Features: marker(s): ampR, tetR, URA3
replicon: pMB1
terminator: none
enhancer: none
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
YI-type integrating shuttle vector.
This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 from lambda590-Sc2904 into the AvaI site of pBR322. The 5' - 3' orientation of URA3 is clockwise; SmaI and AvaI sites in the 3' of URA3 are close to the PvuII site of pBR322.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

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Shipped frozen
Shipping Information Distributed: freeze-dried