YIp31 [pRB22] (ATCC® 37053)

Applications: YI-type (integrating) shuttle vectorintegrating vectorshuttle vector  /  Depositors: D Botstein

Permits and Restrictions

View Permits

Designations YIp31 [pRB22]
Depositors D Botstein
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 5.5000000000000000
Vector: YIp31 (plasmid)
Construction: pBR322, Saccharomyces cerevisiae
Construct size (kb): 5.5
Features: marker(s): ampR, URA3
replicon: pMB1
YI-type (integrating) shuttle vector
integrating vector
shuttle vector
A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector.
This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 into the HindIII site of pBR322. The 5' - 3' orientation of URA3 is clockwise.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C

Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030

Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863

Shipped freeze-dried