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Vector Information
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Size (kb): 5.5000000000000000 Vector: YIp31 (plasmid) Construction: pBR322, Saccharomyces cerevisiae Marker(s):URA3,ampR Construct size (kb): 5.5 Features: marker(s): ampR, URA3
replicon: pMB1
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Applications
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YI-type (integrating) shuttle vector integrating vector shuttle vector
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Comments
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A general purpose integrating Saccharomyces cerevisiae/Escherichia coli shuttle vector. This was constructed by cloning the 1.1 kb HindIII fragment containing URA3 into the HindIII site of pBR322. The 5' - 3' orientation of URA3 is clockwise.
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References
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Botstein D, et al. Sterile host yeast (SHY): a eukaryotic system of biological containment for recombinant DNA experiments. Gene 8: 17-24, 1979. PubMed: 395030
Parent SA, et al. Vector systems for the expression, analysis, and cloning of DNA sequences in S. cerevisiae. Yeast 1: 83-138, 1985. PubMed: 3916863
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