pKT279 (ATCC® 37035)

Applications: expression vectorvector with sequences for protein export  /  Depositors: WD Gilbert

Designations pKT279
Permits and Restrictions

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Depositors WD Gilbert
Biosafety Level 1
Vector Information
Size (kb): 3.7999999523162840
Vector: pKT279 (plasmid)
Promoters: Promoter amp
Construction: pBR322
Construct size (kb): 3.799999952316284
Features: marker(s): tetR
promoter: amp
replicon: pMB1
terminator: none
enhancer: none
expression vector
vector with sequences for protein export
PstI cloning site distal to pre-penicillinase signal sequence.
Vector for secretion of cloned gene product.
Media ATCC® Medium 1273: LB medium (ATCC medium 1065) with 20 mcg/ml tetracycline
Growth Conditions
Temperature: 37.0°C

Talmadge K, Gilberg W. Construction of plasmid vectors with unique PstI cloning sites in a signal sequence coding region. Gene 12: 235-241, 1980. PubMed: 6265317

Shipped freeze-dried