pC194 (ATCC® 37034)

Other IDs:

Nucleotide (GenBank) : VB0132 pC194 cloning vector for Bacillus subtillis.

 /  Applications: shuttle vector  /  Depositors: D Dubnau

Permits and Restrictions

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Designations pC194
Other IDs

Nucleotide (GenBank) : VB0132 pC194 cloning vector for Bacillus subtillis.

Depositors D Dubnau
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Vector Information
Size (kb): 2.9000000953674320
Vector: pC194 (plasmid)
Construction: natural isolate
Construct size (kb): 2.900000095367432
Features: marker(s): cmlR
replicon: pC194
shuttle vector
natural isolate
General purpose plasmid vector for cloning in Bacillus.
Media ATCC® Medium 1675: LB medium (ATCC medium 1065) with 10 mcg/ml Chloramphenicol
Growth Conditions
Temperature: 37.0°C

Gryczan TJ, et al. Characterization of Staphylococcus aureus plasmids introduced by transformation into Bacillus subtilis. J. Bacteriol. 134: 318-329, 1978. PubMed: 418061

Ehrlich SD. DNA cloning in Bacillus subtilis. Proc. Natl. Acad. Sci. USA 75: 1433-1436, 1978. PubMed: 418413

Horinouchi S, Weisblum B. Nucleotide sequence and functional map of pC194, a plasmid that specifies inducible chloramphenicol resistance. J. Bacteriol. 150: 815-825, 1982. PubMed: 6950931

Shipped frozen
Shipping Information

Shipped:  Frozen glycerol stock of B. subtilis containing the plasmid.