pPLc28 [PL-C, pST28] (ATCC® 31696)

Applications: expression vector  /  Depositors: Biogen, Inc., JF Haley, Biogen, Inc.

Permits and Restrictions

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Designations pPLc28 [PL-C, pST28]
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished.
Depositors Biogen, Inc., JF Haley, Biogen, Inc.
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Host
Distribution host: Escherichia coli deltaH1; K-12 delta H1
Distribution host: Escherichia coli deltaH1; K-12 delta H1
Vector Information
Size (kb): 2.7200000286102300
Vector: pPLc28 (plasmid)
Promoters: Promoter lambda PL
Construction: pBR322, lambda
Marker(s):ampR
Construct size (kb): 2.72000002861023
Features: marker(s): ampR
promoter: lambda PL
replicon: pMB1
enhancer: none
Applications
expression vector
Comments
Restriction digests of the clone give the following sizes (kb): EcoRI--2.7; BamHI--2.7; HindIII--2.7.
The following unique restriction sites are found on this vector separated by (bp)(approx): BglI- 100- PstI- 150- PvuI- 1020- EcoRI- 10- BamHI-HindIII-1420.
Plates equally well at 28C and 42C in E. coli K-12 deltaH1 hosts.
Escherichia coli K-12 deltaH1 is K-12 M72 lac(am) deltatrpEA2 rpsL lambda cI857 N(am7)N(am53) deltaH1 bio-. deltaH1 removes part of cro and all genes to the right of cro.
Shows reduced plating efficiency in E. coli M5219 at 42C under antibiotic selection.
This vector is used for expression of fragments containing a ribosome binding site.
The orientation of the PL promoter is clockwise with respect to the plasmid ori.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 28.0°C
References

Fiers WC, Remaut ER. Vectors and methods for making such vectors and for expressing cloned genes. US Patent 4,874,702 dated Oct 17 1989

Remaut E, et al. Plasmid vectors for high-efficiency expression controlled by the PL promoter of coliphage lambda. Gene 15: 81-93, 1981. PubMed: 6271633

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