pGH6 (ATCC® 31539)

Applications: expression vector  /  Depositors: Genentech, Inc.

Designations pGH6
Permits and Restrictions

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Depositors Genentech, Inc.
U.S. Patent
Disclosure This material is cited in a US or other Patent and may not be used to infringe the claims. Depending on the wishes of the Depositor, ATCC may be required to inform the Patent Depositor of the party to which the material was furnished. This material may not have been produced or characterized by ATCC.
Biosafety Level 1
Host
Distribution host: Escherichia coli 294; GMBH K-12 294
Distribution host: Escherichia coli 294; GMBH K-12 294
Vector Information
Size (kb): 4.5999999046325680
Vector: pGH6 (plasmid)
Promoters: Promoter lacUV5
Construction: pBR322, pKB268
Marker(s):ampR,tetR
Construct size (kb): 4.599999904632568
Features: marker(s): ampR, tetR
promoter: lacUV5
replicon: pMB1
Applications
expression vector
Comments
pGH6 is available in a bacteria culture as ATCC 31539 or as purified plasmid DNA as ATCC 40012.
Expression vector. The EcoRI site is downstream of the lacUV5 promoters.
This was constructed by inserting a 285 bp EcoRI fragment from pKB268 (containing 2 95 bp lac UV5 promoters separated by heterologous DNA) into the EcoRI site of pBR322. The EcoRI site distal to the tetR gene was destroyed.
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Growth Conditions
Temperature: 37.0°C
Shipped freeze-dried
Shipping Information Distributed: freeze-dried