Escherichia coli (Migula) Castellani and Chalmers (ATCC® 47055)

Strain Designations: S17-1  /  Type Strain: no  /  Biosafety Level: 1

Classification Enterobacteriaceae, Escherichia
Strain Designations S17-1
transformation host
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Product Format frozen
Storage Conditions -80°C or colder
Preceptrol® no
Genotype recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 integrated into the chromosome
Type Strain no
Recommended mobilization host for pARO180, pARO190, pARO181, and pARO191 (ATCC 77123-77126, respectively) because it contains chromosomally integrated tra genes.
A kanamycin-sensitive strain devoid of the E. coli K-12-specific restriction system allowing efficient uptake of foreign DNA.
The properties of streptomycin, trimethoprim, and spectinomycin resistance were verified.
RP4-Tc::Mu-Km::Tn7 is integrated into the chromosome.
Medium ATCC® Medium 2928: M9 + 40 ug/ml Proline + 10 ug/ml Trimetropin
Growth Conditions
Temperature: 37°C
Atmosphere: Aerobic
Genotype recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 integrated into the chromosome
Name of Depositor D Parke, Department of Biology, Yale University, New Haven, CT
Chain of Custody
ATCC <-- D Parke <-- R Simon

McFarlane GJ, et al. A simplified method for conjugal gene transfer into the filamentous cyanobacterium Anabaena sp. ATCC 27893. J. Microbiol. Methods 6: 301-305, 1987.

Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423

Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983.