VMTRC49 (ATCC® 87764)

Organism: Cryptosporidium sp.  /  Clone Type: Library  /  Depositors: NV Khramtsov, SJ Upton

Permits and Restrictions

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Designations VMTRC49
Species Cryptosporidium sp.
Depositors NV Khramtsov, SJ Upton
Origin
Cryptosporidium andersoni oocysts/sporozoites
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Vector
Tissue: Cryptosporidium andersoni oocysts/sporozoites
DNA: genomic
Digest: Complete
Insert size range: 0.6-7.0 kb
Coverage: Clones for "complete" coverage: 30000.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: Frozen
Comments
The VMTRC49 isolate of Cryptosporidium andersoni was collected from the feces of a Holstein cow, Bos taurus, in August, 1998 near Tulare, CA. Oocysts for DNA extraction were submitted by Dr. E.R. Atwill at University of California-Davis.
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