95-400 Idaho - 2 (ATCC® 87716)

Organism: Cryptosporidium sp.  /  Clone Type: Library  /  Depositors: SJ Upton, NV Khramtsov

Permits and Restrictions

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Designations 95-400 Idaho - 2
Species Cryptosporidium sp.
Depositors SJ Upton, NV Khramtsov
Origin
sporozoites/oocysts
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Vector
Tissue: sporozoites/oocysts
DNA: genomic
Digest: Complete
Insert size range: 0.2 - 2.0
Coverage: Clones for "complete" coverage: 16000.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen
Comments
The 95-400 isolate was collected from the feces of a two year old beef steer in Ada County, Idaho. Oocysts were purified from feces on CsCl gradients and surface sterilized with 10% Clorox prior to nucleic acid extraction.
References

Nikolai V Khramtsov, personal communication

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