KSU-1 (ATCC® 87439)

Organism: Cryptosporidium parvum Tyzzer  /  Clone Type: Library  /  Depositors: NV Khramtsov, SJ Upton

Designations KSU-1
Species Cryptosporidium parvum Tyzzer
Depositors NV Khramtsov, SJ Upton
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: pBluescript II SK+
Intact vector size: 2.961
Type of vector: phagemid
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Polylinker sites: BssHII KpnI ApaI DraII XhoI HincII AccI SalI ClaI HindIII
EcoRI PstI SmaI BamHI XbaI NotI EagI SacI
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ'
marker(s): ampR
replicon: pMB1, f1
Cross references:
Vector
DNA: genomic
Digest: Complete
Insert size range: 0.2-5.0 Kb
Coverage: Clones for "complete" coverage: 15000.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.4 mL)
Comments
The KSU-1 isolate of cryptosporidium parvum was isolated from the feces of naturally infected calf (Bos taurus) in 1987. The isolate was passaged 6-10 times per year by oral inoculation of goats, mice and/or calves prior to library construction in 1992.
Approximately 1% of the recombinants in this library contain two ligated EcoRI inserts.
References

Khramtsov NV, et al. Cloning and analysis of a Cryptosporidium parvum gene encoding a protein with homology to cytoplasmic form hsp70. J. Eukaryot. Microbiol. 42: 416-422, 1995. PubMed: 7620467

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