2Q5 (ATCC® 87409)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: F Kao

Permits and Restrictions

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Designations 2Q5
Species Homo sapiens, human
Depositors F Kao
Origin
lymphoblast cell line GM03714 (46, XX)
Applications
Microdissection library derived from human chromosome 2. Can be used for chromosome painting and for isolating region specific probes.
Vector
Tissue: lymphoblast cell line GM03714 (46, XX)
Chromosome: 2q21-q22
DNA: genomic
Digest: Complete
Insert size range: 50-600 bp
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
Microdissection library derived from human chromosome 2. Can be used for chromosome painting and for isolating region specific probes.
Approximately 50% of the clones contain repetitive sequences. Of 37 single copy clones tested, 26 (70%) hybridized to chromosome 2.
Constructed by microdissecting fragments from 20 chromosomes, treating with proteinase K, extracting with phenol, ligating to an MboI linker-adaptor, amplifying by PCR, digesting with MboI, and ligating to pUC19.
References

Kao FT, et al. Complete set of eleven region-specific microdissection libraries for human chromosome 2. Somatic Cell Mol. Genet. 22: 57-66, 1996. PubMed: 8643994

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