2Q3 (ATCC® 87309)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: F Kao

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Designations 2Q3
Species Homo sapiens, human
Depositors F Kao
lymphoblast cell line GM03714 (46, XX)
Colonies of different sizes may be observed.
Tissue: lymphoblast cell line GM03714 (46, XX)
Chromosome: 2q31-q32
DNA: genomic
Digest: Complete
Insert size range: 50-800 bp
Coverage: Clones for "complete" coverage: 630000.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Colonies of different sizes may be observed.
Region specific library constructed by microdissection of metaphase chromosomes, complete digestion with MboI, followed by PCR amplification and subcloning of amplification products.
Approximately 70% of unique sequences were derived from chromosome 2, as determined by hybridization. Half of all the clones contain repetitive sequence which should be human and chromosome 2 specific.
The library is estimated to be 85% pure.
Approximately half of the clones in the library contain unique or low-copy number inserts.
The mean insert size is 190 bp.

Yu J, et al. Three region-specific microdissection libraries for the long arm of human chromosome 2, regions q33-q35, q31-q32 and q23-q24. Somatic Cell Mol. Genet. 21: 335-343, 1995. PubMed: 8619130