lambdaADH - human B cell cDNA (ATCC® 87286)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations lambdaADH - human B cell cDNA
Species Homo sapiens, human
Depositors SJ Elledge
B lymphocyte (EBV transformed)
Name of vector: lambdaADH
Intact vector size: 42.400
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI EcoRI
Polylinker sites: BglII EcoRI BamHI XhoI BglII
Cloning capacity: 0 - 8.4 kb
Construction: lambdaYES-R, pADH
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: ADH, ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: right arm (15.2 kb, with cI857)
Cross references:
Tissue: B lymphocyte (EBV transformed)
Insert size range: 0.7 - 7 kb
Insert ends: XhoI adaptors
Insert site: XhoI
Number of independent recombinants: 1 x 10^8
Non-recombinants (%): 5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
This has the ADH promoter driving cDNAs, but require their own translational start.