lambdaHA - Saccharomyces cerevisiae cDNA (ATCC® 87281)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations lambdaHA - Saccharomyces cerevisiae cDNA
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors SJ Elledge
Name of vector: lambdaHA
Intact vector size: 42.600
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI, EcoRI
Polylinker sites: EcoRI XhoI EcoRI
Cloning capacity: 0 - 8.0 kb
Construction: lambdaYES-R
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: GAL1, ->
epitope tag: hemagglutinin (HA), ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: lox, ->
other: right arm (15.2 kb, with cI857)
Cross references:
Insert size range: 0.7 - 7 kb
Biosafety Level 1
Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
Inserts in the correct frame will be expressed with a hemagglutinin epitope tag at the amino terminus.
The vector is derived from lambdaYES-R. A sequence providing translation initiatin signals and encoding the epitope tag was inserted between the GAL1 promoter and the cloning site.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

Stephen J Elledge, personal communication

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