lambdaHA - Saccharomyces cerevisiae cDNA (ATCC® 87281)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations lambdaHA - Saccharomyces cerevisiae cDNA
Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors SJ Elledge
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaHA
Intact vector size: 42.600
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI, EcoRI
Polylinker sites: EcoRI XhoI EcoRI
Cloning capacity: 0 - 8.0 kb
Construction: lambdaYES-R
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: GAL1, ->
epitope tag: hemagglutinin (HA), ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: lox, ->
other: right arm (15.2 kb, with cI857)
Cross references:
Vector
DNA: cDNA
Insert size range: 0.7 - 7 kb
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
Comments
Inserts in the correct frame will be expressed with a hemagglutinin epitope tag at the amino terminus.
The vector is derived from lambdaYES-R. A sequence providing translation initiatin signals and encoding the epitope tag was inserted between the GAL1 promoter and the cloning site.
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Stephen J Elledge, personal communication

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