lambdaYES - human B cell cDNA (ATCC® 87273)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Designations lambdaYES - human B cell cDNA
Species Homo sapiens, human
Depositors SJ Elledge
Origin
B lymphocyte (EBV transformed)
Applications
To convert bacteriophage lambda clones to plasmid via the cre-lox recombination system, the use of host strain E. coli BNN 132 (ATCC 47059) is recommended.
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaYES-R
Intact vector size: 42.600
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI, EcoRI
Polylinker sites: EcoRI XhoI EcoRI
Construction: lambdagt6; pSE936
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: GAL1, ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: lox, ->
other: right arm (15.2 kb, with cI857)
Cross references:
Vector
Tissue: B lymphocyte (EBV transformed)
DNA: cDNA
Insert size range: 0.7 - 7 kb
Insert size range (kb): 0.7 - 7
Insert ends: XhoI adaptors
Insert site: XhoI
Number of independent recombinants: 1 x 10^8
Non-recombinants (%): 5
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen bacteria-free lysate (volume: 0.4 mL)
Comments
To convert bacteriophage lambda clones to plasmid via the cre-lox recombination system, the use of host strain E. coli BNN 132 (ATCC 47059) is recommended.
References

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

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