FP4 (ATCC® 77434)

Organism: Homo sapiens, human  /  Clone Type: Library  /  Depositors: A Swaroop

Permits and Restrictions

View Permits

Designations FP4
GenBank Definition

Homo sapiens DPH2L mRNA, complete cds.

GenBank Number

U34880

Species Homo sapiens, human
Depositors A Swaroop
Origin
fetus, 9 week
Vector
Tissue: fetus, 9 week
DNA: cDNA
Insert size range: > 0.8 kb
Genome Equivalent: 0.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Comments
cDNA synthesis was primed by oligo-dT, and a synthetic oligonucleotide (5'GCTTGAATTCAAGC 3') was added for directional cloning.
References

Swaroop A. Construction of directional cDNA libraries from human retinal tissue/cells and their enrichment for specific genes using an efficient subtraction procedure. Methods Neurosci. 15: 285-300, 1993.

Swaroop A, Xu J. cDNA libraries from human tissues and cell lines. Cytogenet. Cell Genet. 64: 292-294, 1993. PubMed: 8404057

Swaroop A, Weissman SM. Charon BS(+) and (-), versatile lambda phage vectors for constructing directional cDNA libraries and their efficient transfer to plasmids. Nucleic Acids Res. 16: 8739, 1988. PubMed: 2971160

Product Sheet
Product Sheet
Product Sheet