rat kidney (ATCC® 77401)

Organism: Rattus norvegicus, rat  /  Clone Type: Library  /  Depositors: JC Edman

Permits and Restrictions

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Designations rat kidney
Species Rattus norvegicus, rat
Depositors JC Edman
Origin
kidney, male
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdagt11
Intact vector size: 43.700
Type of vector: phage
Vector end: EcoRI
Vector end: EcoRI
Cloning sites: EcoRI
Polylinker sites:
Cloning capacity: 7.2 kb
Construction: lambdagt7-lac5(b522 nin5); lambdagt4(cI857 S100 nin5)
Host range: Escherichia coli
Features (with orientation and position when available):
insert detection: lacZ
promoter: lac
replicon: lambda
repressor gene: cI857
Cross references:
Vector
Tissue: kidney, male
DNA: cDNA
Insert size range: 0.6 - 9 kb
Genome Equivalent: 0.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 mL)
Comments
Constructed after size selection of cDNAs. Internal EcoRI sites were methylated and inserts were cloned using EcoRI (8mer) linkers.
References

Morgan DO, et al. Insulin-like growth factor II receptor as a multifunctional binding protein. Nature 329: 301-307, 1987. PubMed: 2957598

Jeffrey C Edman, personal communication

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