E171 (ATCC® 77310)

Organism: Mus musculus, mouse  /  Clone Type: Library  /  Depositors: JL Rubenstein

Designations E171
Species Mus musculus, mouse
Depositors JL Rubenstein
telencephalon, embryonic day 15
Name of vector: E61
Intact vector size: 3.800
Type of vector: phagemid
Vector end: EcoRV
Vector end: NotI
Cloning sites: SacI XhoI XbaI SfiI SacII NotI EcoRI EcoRV HindIII ClaI SalI
Polylinker sites: SacI XhoI XbaI SfiI SacII NotI - stuffer - EcoRI EcoRV
HindIII ClaI SalI
Construction: pBluescript SK-
Host range: Escherichia coli
Features (with orientation and position when available):
marker(s): ampR
promoter: lac, T3, T7
replicon: pMB1, f1
Cross references:
Tissue: telencephalon, embryonic day 15
Digest: Complete
Insert size range: 0.3 - 10 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 100000.0
Media ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 mL)
The vector is a derivative of pBluescript SK- modified in the polylinker to minimize complementarity between single-stranded forms of SK- and KS- plasmids.
In this vector, transcription from the T7 promoter gives the sense strand.
Directionally cloned library.
Classification Eukaryota, Animalia, Metazoa, Chordata, Vertebrata, Tetrapod

Porteus MH, et al. Isolation and characterization of a library of cDNA clones that are preferentially expressed in the embryonic telencephalon. Brain Res. Mol. Brain Res. 12: 7-22, 1992. PubMed: 1372074

Rubenstein JL, et al. Subtractive hybridization system using single-stranded phagemids with directional inserts. Nucleic Acids Res. 18: 4833-4842, 1990. PubMed: 2168539