(ATCC® 77256)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

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Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors SJ Elledge
Name of vector: lambdaYES-R
Intact vector size: 42.600
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI, EcoRI
Polylinker sites: EcoRI XhoI EcoRI
Construction: lambdagt6; pSE936
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: GAL1, ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: lox, ->
other: right arm (15.2 kb, with cI857)
Cross references:
DNA: genomic
Digest: Sheared
Insert size range: 4.0 - 8.0 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 3000.0
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Shipping Information Distributed: frozen (volume: 0.2 mL)
The genotype of FNY243 is leu2-04 ade1 ade6 cir0.
A random shear library amplified once by the depositor.
Can be converted into plasmids by infection into E. coli BNN132 (ATCC 47059).
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

Stephen J Elledge, personal communication