(ATCC® 77256)

Organism: Saccharomyces cerevisiae Meyen ex E.C. Hansen  /  Clone Type: Library  /  Depositors: SJ Elledge

Permits and Restrictions

View Permits

Species Saccharomyces cerevisiae Meyen ex E.C. Hansen
Depositors SJ Elledge
Vector
DESCRIPTION OF VECTOR COMPONENT:
Name of vector: lambdaYES-R
Intact vector size: 42.600
Type of vector: phage
Vector end: XhoI
Vector end: XhoI
Cloning sites: XhoI, EcoRI
Polylinker sites: EcoRI XhoI EcoRI
Construction: lambdagt6; pSE936
Host range: Escherichia coli
Features (with orientation and position when available):
other: left arm (19.6 kb), ->
other: lox, ->
replicon: ARS1
replicon: pMB1
marker(s): ampR, <-
promoter for expression: GAL1, ->
MCS: XhoI EcoRI, ->
promoter for expression: lac, <-
transcription terminator: HIS3, ->
marker(s): URA3, ->
centromere: CEN4
other: lox, ->
other: right arm (15.2 kb, with cI857)
Cross references:
Vector
DNA: genomic
Digest: Sheared
Insert size range: 4.0 - 8.0 kb
Genome Equivalent: 0.0
Coverage: Clones for "complete" coverage: 3000.0
Biosafety Level 1
Shipping Information Distributed: frozen (volume: 0.2 mL)
Comments
The genotype of FNY243 is leu2-04 ade1 ade6 cir0.
A random shear library amplified once by the depositor.
Can be converted into plasmids by infection into E. coli BNN132 (ATCC 47059).
Classification Saccharomycetes, Saccharomycetidae, Saccharomycetales, Saccharomycetaceae, Saccharomycetaceae, Saccharomyces, cerevisiae
References

Elledge SJ, et al. lambdaYES: a multifunctional cDNA expression vector for the isolation of genes by complementation of yeast and Escherichia coli mutations. Proc. Natl. Acad. Sci. USA 88: 1731-1735, 1991. PubMed: 1848010

Stephen J Elledge, personal communication

Product Sheet
Product Sheet
Product Sheet